Beckman coulter ultracentrifuges have a history of over 70 years were known affectionately as “Spincos” and constantly delivered first leading edge technology, since first developing the Model E, the world’s first analytical ultracentrifuge in 1947. From first floor-type preparative ultracentrifuge in 1949, first vacuum encased induction drive, first table-top ultracentrifuge and first electric non-chlorofluorocarbon (CFC) thermoelectric heating and cooling system. Accompany with first titanium rotor, top-loading swing rotor and near-vertical rotor.

  Ultracentrifugation has been a classic important application in disease control for harvesting of virus or virus preparation or virus purification in density gradients or sucrose gradient from cell culture medium to preparation of viruses for Electron Microscopy, to more exotic applications like the quantification of viruses.

  Ultracentrifugation can be used for separation of organelles and other subcellular components based on their density and size. It can be used for purification of macromolecules such as proteins, nucleic acids, and polysaccharides and to study macromolecular interactions, such as protein-protein and protein-DNA interactions. By analyzing sedimentation velocity or equilibrium data from ultracentrifuge, the molecular weight, shape, and stoichiometry of macromolecules can be determined. Ultracentrifugation can be used to separate lipoproteins such as Chylomicrons based on their density, fractionation of lipoproteins which is useful for studying lipid metabolism such as those measuring cholesterol or triglycerides and assessing cardiovascular disease risk.

  Modern applications include to use differential ultracentrifugation for exosomes concentration or exosomes purification, to eliminate bovine exosomes from media. Exosomes are small membrane-bound vesicles secreted by cells that can be present in cell culture media, including fetal bovine serum (FBS)-supplemented media fetal bovine serum incorporated media. Another application would be concentration of lentiviral particles using sucrose cushion or Phosphate Buffered Saline. Lentiviruses are commonly used viral vectors for genetic manipulation in mammalian cells. Analyze the concentrated lentiviral particles for titer and purity using methods such as qPCR or ELISA.

  Current model available from Beckman Coulter are the premier ultracentrifuge model Optima™ XPN and tabletop Optima MAX-XP security and tracking features to ensure chain of custody and compliance.The simplifies Optima XE and tabletop Optima MAX-TL. The Optima XPN with networking capability and customizable offers multi-layered BioSafety* features for a safe and productive work environment. The unit is equipped with the innovative eXpert simulation software and other safety system incoroperated such as vacuum-encased drive unit, regenerative braking system, magnetic sensor rotation monitoring function, optical sensor overspeed disk and dynamic rotor inertia check. The Airfuge Air-Driven ultracentrifuge offers a simple, rapid method for removing chylomicrons (fat particles in lipemic samples) for accurate lipemic clarification results.

  The Labware such as OptiSeal Tubes, QuickSeal Tubes, g-MAX System and Konical Tubes was designed to optimizing performance, ease of use, and safety for application for vaccine strain preparation such as nucleic acid RNA/DNA, Protein (mAb) and multi-antigen.

  Beckman Coulter centrifuges are Malaysian Medical Device Authority (MDA) registered centrifuge.