Beckman Coulter high-speed centrifuges are versatile tools for simple pelleting to other techniques to get the best results. Those techniques are rate zonal for separation based on size & shape; elutriation for separation based on size and density; and continuous flow separations similar as elutriation but higher volumes of pelleting required.

  Equipped with the exclusive high torque, switched reluctance (SR) drive that shortens cycle times. Beckman coulter High Speed Centrifuge Can accelerate sample processing with min 21,000 RMP and with the maximum 110,510 g-force. Simplified high throughput sample processing workflows and ergonomic design to saving lab space and MobileFuge remote application overcoming shared lab challenges.

  Beckman Coulter Avanti series of high-speed centrifuges are uniquely designed for your optimized production needs. Dynamic rotor inertia check and biosafe safety feature to prevent accidents and to protect the safety of the user. And for sustainable feature, build-in Friction Reduction System (FRS) which reduce the rotor chamber to one-fourth of atmospheric pressure, increases total centrifuge reliability by reducing the workload of the centrifuge’s two major mechanical components, the drive and refrigeration systems. By reducing the friction or drag inherent in a rotating system, it will decrease heat generation and increase centrifugal acceleration. The improved temperature control for rotors will improve energy efficiency thus lower the operating cost and extended the centrifuge life.

  Centrifuge most used at any proteomics laboratory for protein purification and protein aggregation qualification. Using various proteomics applications with Avanti high-speed centrifuges and fixed angle large volume processing, lower speed rotor for cell pelleting or cell harvesting the step is called harvest and lyse in step 1. If higher volumes of pelleting required, continuous flow separations rotor as an option. The next step is efficient separation of protein from non-protein components or protein precipitation and cellular debris or called lysate clarification – step 2 with a high-speed, low volume rotor. Next step 3 will be crude purification, using precipitants like ammonium sulfate, polyethylene glycol, etc. To recover the bulk protein from a crude extract and are used as primary purification methods. The separation of these precipitates requires medium speeds and low- to medium-volume fixed angle rotors or Beckman coulter benchtop centrifuge can be use too. Chromatography columns Step 4, or secondary purification will be next and again Beckman Coulter Benchtop Microcentrifuge help purify small quantities of recombinant proteins in a fast and simple manner. Step 5 tertiary purification , subcellular and membrane isolation such as mitochondria, plasma membranes, etc. using density gradients or continuous rate zonal technique for purified proteins or protein concentration. The last step 6 will be concentration and buffer exchange. The concentration and right buffer of the pure protein are a must. Once the debris has been removed, the sample is subjected to Beckman Coulter ultracentrifugation to separate the individual components of the sample based on their density. Then high-speed centrifuge will be centrifugal concentrator as the protein of interest may be present in a diluted form. There are various filter-based columns available which are used for buffer exchange. Beckman Coulter high-speed centrifuge have multiple adapters available for these spin columns concentrators.

  Protein purification can be broadened to many areas such as cell separation/cell lyse/cell pelleting/cell harvesting, separating bacterial/yeast, virus purification and DNA and RNA isolation for downstream applications.

  Not only high-speed centrifugation is a powerful tool for separation, purification, and analysis of biological sample, it can be used in the synthesis of nanoparticles for drug delivery, imaging, and other applications. And is widely used in industries such as biopharmaceuticals, food and beverage, and wastewater treatment for separation and purification of various products. Examples of nanoparticle synthesis are Liposomes nanoparticles for drug delivery, gold & iron oxide nanoparticles for imaging and drug targeting applications. High-speed centrifugation is widely used in wastewater treatment for separation and purification of various products, including sludge dewatering, oil-water separation, and removal of suspended solids.

  Current model available from Beckman Coulter are the premier high-speed model Avanti JXN, the simplifies Avanti J-E Series and high-capacity Avanti J-HC with labware which includes Harvest Liners to minimize contamination, convenient sample recovery and long-term sample storage .

  Beckman Coulter centrifuges are Malaysian Medical Device Authority (MDA) registered centrifuge.